1- Department of Veterinary Medicine, Sho.C., Islamic Azad University, Shoushtar, Iran
2- Department of Veterinary Medicine, Sho.C., Islamic Azad University, Shoushtar, Iran ,morteza_vet@yahoo.com
3- Department of Veterinary Medicine, Be.C., Islamic Azad University, Behbahan, Iran
2- Department of Veterinary Medicine, Sho.C., Islamic Azad University, Shoushtar, Iran ,
3- Department of Veterinary Medicine, Be.C., Islamic Azad University, Behbahan, Iran
Abstract: (348 Views)
Background: Second-degree burns present significant therapeutic challenges because of prolonged inflammation and impaired regeneration. This study compared the healing efficacy of an Artemisia sieberi essential oil nanoemulsion with that of conventional Alpha ointment by assessing morphological indices and cytokine gene expression in a rat model.
Methods: In this experimental study, 21 male Wistar rats were randomly assigned to control, Alpha ointment, and Artemisia nanoemulsion groups. Following the induction of standardized second-degree thermal burns, healing was monitored over 21 days. Wound surface area was measured macroscopically, whereas histopathological architecture, including re-epithelialization and collagen deposition, was assessed using H&E staining. Furthermore, the expression levels of IL-6, TNF-α, and IL-10 in healed tissues were quantified using RT-qPCR. Data were analyzed using one-way ANOVA and Tukey’s post-hoc test (P- Value <0.05).
Results: By day 21, the Artemisia nanoemulsion group exhibited a significantly higher wound-contraction rate than the Alpha and control groups (P<0.001). Histopathological examination confirmed superior re-epithelialization and organized fibroblast proliferation in nanoemulsion-treated tissues. At the molecular level, this treatment induced significant downregulation of pro-inflammatory cytokines (IL-6 and TNF-α) and marked upregulation of the anti-inflammatory cytokine (IL-10), demonstrating greater efficacy than Alpha ointment in modulating the local immune response.
Conclusion: Artemisia sieberi nanoemulsion effectively accelerates burn healing by suppressing inflammation and promoting tissue remodeling, thereby representing a promising therapeutic agent for the clinical management of burns.
Methods: In this experimental study, 21 male Wistar rats were randomly assigned to control, Alpha ointment, and Artemisia nanoemulsion groups. Following the induction of standardized second-degree thermal burns, healing was monitored over 21 days. Wound surface area was measured macroscopically, whereas histopathological architecture, including re-epithelialization and collagen deposition, was assessed using H&E staining. Furthermore, the expression levels of IL-6, TNF-α, and IL-10 in healed tissues were quantified using RT-qPCR. Data were analyzed using one-way ANOVA and Tukey’s post-hoc test (P- Value <0.05).
Results: By day 21, the Artemisia nanoemulsion group exhibited a significantly higher wound-contraction rate than the Alpha and control groups (P<0.001). Histopathological examination confirmed superior re-epithelialization and organized fibroblast proliferation in nanoemulsion-treated tissues. At the molecular level, this treatment induced significant downregulation of pro-inflammatory cytokines (IL-6 and TNF-α) and marked upregulation of the anti-inflammatory cytokine (IL-10), demonstrating greater efficacy than Alpha ointment in modulating the local immune response.
Conclusion: Artemisia sieberi nanoemulsion effectively accelerates burn healing by suppressing inflammation and promoting tissue remodeling, thereby representing a promising therapeutic agent for the clinical management of burns.
Keywords: Burns, Nanoemulsion (NB-201 nanoemulsion [Supplementary Concept]), Cytokines, Wound Healing, Artemisia Sieberi
Editorial: Original article |
Subject:
Basic Medical Sciences
Received: 2026/04/10 | Accepted: 2026/05/31
Received: 2026/04/10 | Accepted: 2026/05/31
References
1. Hamrahi V, Hamblin MR, Carter EA, Benjamin J, Francis KP, Fischman AJ, et al. Role of interleukin 6 (IL-6) and tumor necrosis factor (TNF) in burn eschar bacterial colonization and wound healing. J Burn Care Res. 2006;27(Suppl):111. [View at Publisher] [DOI] [Google Scholar]
2. Singer AJ, Dagum AB. Current management of acute cutaneous wounds. N Engl J Med. 2008;359(10):1037-46. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol. 2006 May;71(10):1397-421. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017;9(6):7204-18. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Gava JS, Simões GA, Portes DB, Cruz JV, Maddaleno AS, Vinardell MP, et al. Nanoemulsification Enhances the Regenerative and Safety Profile of Ocimum gratissimum Essential Oil In Vitro. ACS Omega. 2025;10(39):45596-607. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Peranteau WH, Zhang L, Muvarak N, Badillo AT, Radu A, Zoltick PW, et al. IL-10 overexpression decreases inflammatory mediators and promotes regenerative healing in an adult model of scar formation. J Invest Dermatol. 2008;128(7):1852-60. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Sun Z-L, Feng Y, Zou M-L, Zhao B-H, Liu S-Y, Du Y, et al. Emerging role of IL-10 in hypertrophic scars. Front Med (Lausanne). 2020;7:438. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Eming SA, Martin P, Tomic-Canic M. Wound repair and regeneration: Mechanisms, signaling, and translation. Sci Transl Med. 2014;6(265):265sr6. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Ghasemi G, Alirezalu A, Ishkeh SR, Ghosta Y. Phytochemical properties of essential oil from Artemisia sieberi Besser (Iranian accession) and its antioxidant and antifungal activities. Nat Prod Res. 2021;35(21):4154-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Abubakar IB, Loh H-S. A review on ethnobotany, pharmacology and phytochemistry of Tabernaemontana corymbosa. J Pharm Pharmacol. 2016;68(4):423-32. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Preeti, Sambhakar S, Malik R, Bhatia S, Al Harrasi A, Rani C, et al. Nanoemulsion: An emerging novel technology for improving the bioavailability of drugs. Scientifica (Cairo). 2023;2023:6640103. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Chhabra J, Chopra H, Pahwa R, Raina N, Wadhwa K, Saini S, et al. Potential of nanoemulsions for accelerated wound healing: Innovative strategies. Int J Surg. 2023;109(8):2365-77. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Mahmoud NN, Hamad K, Al Shibitini A, Juma S, Sharifi S, Gould L, et al. Investigating inflammatory markers in wound healing: Understanding implications and identifying artifacts. ACS Pharmacol Transl Sci. 2024;7(1):18-27. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Joshi C, Shukla K, Odedra KN, Jadeja BA. Lawsonia inermis L. (Henna): a comprehensive review of its phytochemistry, pharmacological potential, traditional uses, and commercial applications. J Plant Biota. 2025;4(1):99-110. [View at Publisher] [DOI] [Google Scholar]
15. Naseri S, Golpich M, Roshancheshm T, Joobeni MG, Khodayari M, Noori S, et al. The effect of henna and linseed herbal ointment blend on wound healing in rats with second-degree burns. Burns. 2021;47(6):1442-50. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Sanei-Dehkordi A, Ghasemian A, Zarenezhad E, Qasemi H, Nasiri M, Osanloo M. Nanoliposomes containing three essential oils from the Artemisia genus as effective larvicides against Aedes aegypti and Anopheles stephensi. Sci Rep. 2023;13(1):11002. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Khazania B, Almasi H, Mohtarami F, Amjadi S. Incorporation of Artemisia essential oil loaded chitosomes in salep based film for use in toast bread packaging: New generation of active films. Food Packag Shelf Life. 2024;43:101305. [View at Publisher] [DOI] [Google Scholar]
18. Bahloul B, Ben Bnina E, Hamdi A, Castillo Henríquez L, Baccar D, Kalboussi N, et al. Investigating the wound-healing potential of a nanoemulsion-gel formulation of Pituranthos tortuosus essential oil. Gels. 2024;10(3):155. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Wang H, Liu Y, Cai K, Zhang B, Tang S, Zhang W, et al. Antibacterial polysaccharide-based hydrogel dressing containing plant essential oil for burn wound healing. Burns Trauma. 2021:9:tkab041. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Paydar S, Akrami M, Dehghanian A, Alavi Moghadam R, Heidarpour M, Bahari Khoob A, et al. A comparison of the effects of Alpha and medical-grade honey ointments on cutaneous wound healing in rats. J Pharm (Cairo). 2016;2016:9613908. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-29. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Al-Otibi F, Alshammry NA, Alharbi RI, Bin-Jumah MN, AlSubaie MM. Silver nanoparticles of Artemisia sieberi extracts: Chemical composition and antimicrobial activities. Plants (Basel). 2023;12(11):2093. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Zangeneh MM, Zangeneh A, Etemadi N, Tabatabaei Aghda SJ, Pourkamalzadeh M, Zand S, et al. The effect of nano silver particles of the aqueous extract of Artemisia aucheri on the healing process of skin wounds in rats. Pbp. 2023;5(1):1-7. [View at Publisher] [DOI] [Google Scholar]
24. Mahboubi M. Artemisia sieberi Besser: A review of its phytochemistry, pharmacological potential, and traditional uses. J Ethnopharmacol. 2017;200:135-46. [DOI] [PMID]
25. Doroodgar A, Arbabi M, Razavi MR, Mohebali M, Sadr F, Tashakkor Z. Effect of Artemisia sieberi extract on Leishmania major ulcers in BALB/c mice. Feyz Med Sci J. 2007;11(3):52-6. [View at Publisher] [Google Scholar]
26. Mohtasham Amiri Z, Tanideh N, Seddighi A, Mokhtari M, Amini M, Shakouri Partovi A, et al. The effect of Lithospermum officinale, silver sulfadiazine and Alpha ointments in healing of burn wound injuries in rat. World J Plast Surg. 2017;6(3):313-8. [View at Publisher] [PMID] [Google Scholar]
27. Ji S, Xiao S, Xia Z. Consensus on the treatment of second-degree burn wounds (2024 edition). Burns Trauma. 2024:12:tkad061. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Mohammed HA, Qureshi KA, Ali HM, Al-Omar MS, Khan O, Mohammed SAA. Bio-evaluation of the wound healing activity of Artemisia judaica L. as part of the plant's use in traditional medicine; phytochemical, antioxidant, anti-inflammatory, and antibiofilm properties of the plant's essential oils. Antioxidants (Basel). 2022;11(2):332. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Benkhaled A, Boudjelal A, Napoli E, Baali F, Ruberto G. Phytochemical profile, antioxidant activity and wound healing properties of Artemisia absinthium essential oil. Asian Pac J Trop Biomed. 2020;10(11):496-505. [View at Publisher] [DOI] [Google Scholar]
30. Hosseini SV, Tanideh N, Kohanteb J, Ghodrati Z, Mehrabani D, Yarmohammadi H. Comparison between Alpha and silver sulfadiazine ointments in treatment of Pseudomonas infections in 3rd degree burns. Int J Surg. 2007;5(1):23-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Daryabeigi R, Heidari M, Hosseini SA, Omranifar M. Comparison of healing time of the 2nd degree burn wounds with two dressing methods of fundermol herbal ointment and 1% silver sulfadiazine cream. Iran J Nurs Midwifery Res. 2010;15(3):97-101. [View at Publisher] [PMID] [Google Scholar]
32. Duda-Madej A, Viscardi S, Grabarczyk M, Topola E, Kozłowska J, Mączka W, et al. Is Camphor the Future in Supporting Therapy for Skin Infections? Pharmaceuticals (Basel). 2024;17(6):715. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Pathak D, Mazumder A. A critical overview of challenging roles of medicinal plants in improvement of wound healing technology. Daru. 2024;32(1):379-419. [View at Publisher] [DOI] [PMID] [Google Scholar]
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